stemspan serum-free expansion medium 09650 (STEMCELL Technologies Inc)
90
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STEMCELL Technologies Inc
stemspan serum-free expansion medium 09650
Stemspan Serum Free Expansion Medium 09650, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serum-free+expansion+medium/stemspan+serum+free+expansion+medium/pm40670787-630-5-12
Average 90 stars, based on 1 article reviews
Stemspan Serum Free Expansion Medium 09650, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serum-free+expansion+medium/stemspan+serum+free+expansion+medium/pm40670787-630-5-12
Average 90 stars, based on 1 article reviews
stemspan serum-free expansion medium 09650 - by Bioz Stars,
2026-09
90/100 stars
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Cell Culture:Article Title: Dynamic assessment of myelofibrosis progression in myeloproliferative neoplasm mouse model using a minimally invasive evaluation system. Article Snippet: Myelofibrosis (MF) is the most common complication of myeloproliferative neoplasms (MPNs),which is markedly correlated with a dismal prognosis.. Murine model such as the thrombopoietin receptor (MPL)W515Lmutant MPN mouse model functions as a crucial vehicle in disease research, is widely used in basic and applied research on MPNs and MF.However, the lack of methods for dynamic observation of MF progression hinders mechanistic studies and drug development for MF.. Here we develop a sensitive, stable and minimally invasive MPN evaluation system to assess the evolution of myelofibrosis in murine model.Key peripheral blood parameters (WBC, RBC, HGB, HCT, PLT, and tumor cell proportion) from MPN mice were analyzed using PCA to reduce dimensionality and generate a comprehensive evaluation score (Y). Article Title: Increased tumor-associated mast cells facilitate thyroid cancer progression by inhibiting CD8 + T cell function through galectin-9 Article Snippet: For the first 6 weeks, CD133 + cells were cultured in serum-free expansion medium (StemCell, Canada) supplemented with penicillin (100 U/mL)/streptomycin (100 μg/mL), human recombinant (hr) IL-3 (30 ng/mL, only present during the first 3 weeks), hr IL-6 (50 ng/mL), and stem cell factor (SCF) (100 ng/mL). Article Title: MALAT1 promotes platelet activity and thrombus formation through PI3k/Akt/GSK-3β signalling pathway Article Snippet: Article Title: Single-cell lineage capture across genomic modalities with CellTag-multi reveals fate-specific gene regulatory changes Article Snippet: LSK cells were purified as described above, counted and 5,500 cells were added to a 96-well Article Title: NEUROBEACHIN regulates hematopoietic progenitor differentiation and survival by modulating NOTCH activity. Article Snippet: Miguel Ganuza Fernandez (Barts Centre Cancer Research UK, United Kingdom) Antonio Morales-Hernandez (University of Michigan, United States) Alanna Van Huizen (St. Jude Children's Research Hospital, United States) Ashley Chabot (St.Jude Children's Research Hospital, United States) Trent Hall (St. Jude Children's Research Hospital, ) Claire Caprio (St. Jude Children's Research Hospital, United States) David Finkelstein (SJCRH, United States) Shannon McKinney Freeman (St. Jude Children's Research Hospital, United States) Article Title: TOPO-seq reveals DNA topology-induced off-target activity by Cas9 and base editors. Article Snippet: With the increasing use of CRISPR–Cas9, detecting off-target events is essential for safety.. Current methods primarily focus on guide RNA (gRNA) sequence mismatches, often overlooking the impact of DNA topology in regulating off-target activity.. Here we present TOPO-seq, a high-throughput and sensitive method that identifies genome-wide off-target effects of Cas9 and base editors while accounting for DNA topology. Article Title: HSPA9 /mortalin inhibition disrupts erythroid maturation through a TP53 -dependent mechanism in human CD34+ hematopoietic progenitor cells Article Snippet: The cells were subsequently cultured for 5 days in Article Title: Neurobeachin regulates hematopoietic progenitor differentiation and survival by modulating Notch activity Article Snippet: HSPCs were cultured in Recombinant:Article Title: Dynamic assessment of myelofibrosis progression in myeloproliferative neoplasm mouse model using a minimally invasive evaluation system. Article Snippet: Myelofibrosis (MF) is the most common complication of myeloproliferative neoplasms (MPNs),which is markedly correlated with a dismal prognosis.. Murine model such as the thrombopoietin receptor (MPL)W515Lmutant MPN mouse model functions as a crucial vehicle in disease research, is widely used in basic and applied research on MPNs and MF.However, the lack of methods for dynamic observation of MF progression hinders mechanistic studies and drug development for MF.. Here we develop a sensitive, stable and minimally invasive MPN evaluation system to assess the evolution of myelofibrosis in murine model.Key peripheral blood parameters (WBC, RBC, HGB, HCT, PLT, and tumor cell proportion) from MPN mice were analyzed using PCA to reduce dimensionality and generate a comprehensive evaluation score (Y). Article Title: Increased tumor-associated mast cells facilitate thyroid cancer progression by inhibiting CD8 + T cell function through galectin-9 Article Snippet: For the first 6 weeks, CD133 + cells were cultured in serum-free expansion medium (StemCell, Canada) supplemented with penicillin (100 U/mL)/streptomycin (100 μg/mL), human recombinant (hr) IL-3 (30 ng/mL, only present during the first 3 weeks), hr IL-6 (50 ng/mL), and stem cell factor (SCF) (100 ng/mL). Article Title: MALAT1 promotes platelet activity and thrombus formation through PI3k/Akt/GSK-3β signalling pathway Article Snippet: Article Title: Single-cell lineage capture across genomic modalities with CellTag-multi reveals fate-specific gene regulatory changes Article Snippet: LSK cells were purified as described above, counted and 5,500 cells were added to a 96-well Article Title: NEUROBEACHIN regulates hematopoietic progenitor differentiation and survival by modulating NOTCH activity. Article Snippet: Miguel Ganuza Fernandez (Barts Centre Cancer Research UK, United Kingdom) Antonio Morales-Hernandez (University of Michigan, United States) Alanna Van Huizen (St. Jude Children's Research Hospital, United States) Ashley Chabot (St.Jude Children's Research Hospital, United States) Trent Hall (St. Jude Children's Research Hospital, ) Claire Caprio (St. Jude Children's Research Hospital, United States) David Finkelstein (SJCRH, United States) Shannon McKinney Freeman (St. Jude Children's Research Hospital, United States) Article Title: TOPO-seq reveals DNA topology-induced off-target activity by Cas9 and base editors. Article Snippet: With the increasing use of CRISPR–Cas9, detecting off-target events is essential for safety.. Current methods primarily focus on guide RNA (gRNA) sequence mismatches, often overlooking the impact of DNA topology in regulating off-target activity.. Here we present TOPO-seq, a high-throughput and sensitive method that identifies genome-wide off-target effects of Cas9 and base editors while accounting for DNA topology. Article Title: HSPA9 /mortalin inhibition disrupts erythroid maturation through a TP53 -dependent mechanism in human CD34+ hematopoietic progenitor cells Article Snippet: The cells were subsequently cultured for 5 days in Article Title: Neurobeachin regulates hematopoietic progenitor differentiation and survival by modulating Notch activity Article Snippet: HSPCs were cultured in Purification:Article Title: Dynamic assessment of myelofibrosis progression in myeloproliferative neoplasm mouse model using a minimally invasive evaluation system. Article Snippet: Myelofibrosis (MF) is the most common complication of myeloproliferative neoplasms (MPNs),which is markedly correlated with a dismal prognosis.. Murine model such as the thrombopoietin receptor (MPL)W515Lmutant MPN mouse model functions as a crucial vehicle in disease research, is widely used in basic and applied research on MPNs and MF.However, the lack of methods for dynamic observation of MF progression hinders mechanistic studies and drug development for MF.. Here we develop a sensitive, stable and minimally invasive MPN evaluation system to assess the evolution of myelofibrosis in murine model.Key peripheral blood parameters (WBC, RBC, HGB, HCT, PLT, and tumor cell proportion) from MPN mice were analyzed using PCA to reduce dimensionality and generate a comprehensive evaluation score (Y). Article Title: Increased tumor-associated mast cells facilitate thyroid cancer progression by inhibiting CD8 + T cell function through galectin-9 Article Snippet: For the first 6 weeks, CD133 + cells were cultured in serum-free expansion medium (StemCell, Canada) supplemented with penicillin (100 U/mL)/streptomycin (100 μg/mL), human recombinant (hr) IL-3 (30 ng/mL, only present during the first 3 weeks), hr IL-6 (50 ng/mL), and stem cell factor (SCF) (100 ng/mL). Article Title: MALAT1 promotes platelet activity and thrombus formation through PI3k/Akt/GSK-3β signalling pathway Article Snippet: Article Title: Single-cell lineage capture across genomic modalities with CellTag-multi reveals fate-specific gene regulatory changes Article Snippet: LSK cells were purified as described above, counted and 5,500 cells were added to a 96-well Article Title: NEUROBEACHIN regulates hematopoietic progenitor differentiation and survival by modulating NOTCH activity. Article Snippet: Miguel Ganuza Fernandez (Barts Centre Cancer Research UK, United Kingdom) Antonio Morales-Hernandez (University of Michigan, United States) Alanna Van Huizen (St. Jude Children's Research Hospital, United States) Ashley Chabot (St.Jude Children's Research Hospital, United States) Trent Hall (St. Jude Children's Research Hospital, ) Claire Caprio (St. Jude Children's Research Hospital, United States) David Finkelstein (SJCRH, United States) Shannon McKinney Freeman (St. Jude Children's Research Hospital, United States) Article Title: TOPO-seq reveals DNA topology-induced off-target activity by Cas9 and base editors. Article Snippet: With the increasing use of CRISPR–Cas9, detecting off-target events is essential for safety.. Current methods primarily focus on guide RNA (gRNA) sequence mismatches, often overlooking the impact of DNA topology in regulating off-target activity.. Here we present TOPO-seq, a high-throughput and sensitive method that identifies genome-wide off-target effects of Cas9 and base editors while accounting for DNA topology. Article Title: HSPA9 /mortalin inhibition disrupts erythroid maturation through a TP53 -dependent mechanism in human CD34+ hematopoietic progenitor cells Article Snippet: The cells were subsequently cultured for 5 days in Article Title: Neurobeachin regulates hematopoietic progenitor differentiation and survival by modulating Notch activity Article Snippet: HSPCs were cultured in Suspension:Article Title: Dynamic assessment of myelofibrosis progression in myeloproliferative neoplasm mouse model using a minimally invasive evaluation system. Article Snippet: Myelofibrosis (MF) is the most common complication of myeloproliferative neoplasms (MPNs),which is markedly correlated with a dismal prognosis.. Murine model such as the thrombopoietin receptor (MPL)W515Lmutant MPN mouse model functions as a crucial vehicle in disease research, is widely used in basic and applied research on MPNs and MF.However, the lack of methods for dynamic observation of MF progression hinders mechanistic studies and drug development for MF.. Here we develop a sensitive, stable and minimally invasive MPN evaluation system to assess the evolution of myelofibrosis in murine model.Key peripheral blood parameters (WBC, RBC, HGB, HCT, PLT, and tumor cell proportion) from MPN mice were analyzed using PCA to reduce dimensionality and generate a comprehensive evaluation score (Y). Article Title: Increased tumor-associated mast cells facilitate thyroid cancer progression by inhibiting CD8 + T cell function through galectin-9 Article Snippet: For the first 6 weeks, CD133 + cells were cultured in serum-free expansion medium (StemCell, Canada) supplemented with penicillin (100 U/mL)/streptomycin (100 μg/mL), human recombinant (hr) IL-3 (30 ng/mL, only present during the first 3 weeks), hr IL-6 (50 ng/mL), and stem cell factor (SCF) (100 ng/mL). Article Title: MALAT1 promotes platelet activity and thrombus formation through PI3k/Akt/GSK-3β signalling pathway Article Snippet: Article Title: Single-cell lineage capture across genomic modalities with CellTag-multi reveals fate-specific gene regulatory changes Article Snippet: LSK cells were purified as described above, counted and 5,500 cells were added to a 96-well Article Title: NEUROBEACHIN regulates hematopoietic progenitor differentiation and survival by modulating NOTCH activity. Article Snippet: Miguel Ganuza Fernandez (Barts Centre Cancer Research UK, United Kingdom) Antonio Morales-Hernandez (University of Michigan, United States) Alanna Van Huizen (St. Jude Children's Research Hospital, United States) Ashley Chabot (St.Jude Children's Research Hospital, United States) Trent Hall (St. Jude Children's Research Hospital, ) Claire Caprio (St. Jude Children's Research Hospital, United States) David Finkelstein (SJCRH, United States) Shannon McKinney Freeman (St. Jude Children's Research Hospital, United States) Article Title: TOPO-seq reveals DNA topology-induced off-target activity by Cas9 and base editors. Article Snippet: With the increasing use of CRISPR–Cas9, detecting off-target events is essential for safety.. Current methods primarily focus on guide RNA (gRNA) sequence mismatches, often overlooking the impact of DNA topology in regulating off-target activity.. Here we present TOPO-seq, a high-throughput and sensitive method that identifies genome-wide off-target effects of Cas9 and base editors while accounting for DNA topology. Article Title: HSPA9 /mortalin inhibition disrupts erythroid maturation through a TP53 -dependent mechanism in human CD34+ hematopoietic progenitor cells Article Snippet: The cells were subsequently cultured for 5 days in Article Title: Neurobeachin regulates hematopoietic progenitor differentiation and survival by modulating Notch activity Article Snippet: HSPCs were cultured in Virus:Article Title: Dynamic assessment of myelofibrosis progression in myeloproliferative neoplasm mouse model using a minimally invasive evaluation system. Article Snippet: Myelofibrosis (MF) is the most common complication of myeloproliferative neoplasms (MPNs),which is markedly correlated with a dismal prognosis.. Murine model such as the thrombopoietin receptor (MPL)W515Lmutant MPN mouse model functions as a crucial vehicle in disease research, is widely used in basic and applied research on MPNs and MF.However, the lack of methods for dynamic observation of MF progression hinders mechanistic studies and drug development for MF.. Here we develop a sensitive, stable and minimally invasive MPN evaluation system to assess the evolution of myelofibrosis in murine model.Key peripheral blood parameters (WBC, RBC, HGB, HCT, PLT, and tumor cell proportion) from MPN mice were analyzed using PCA to reduce dimensionality and generate a comprehensive evaluation score (Y). Article Title: Increased tumor-associated mast cells facilitate thyroid cancer progression by inhibiting CD8 + T cell function through galectin-9 Article Snippet: For the first 6 weeks, CD133 + cells were cultured in serum-free expansion medium (StemCell, Canada) supplemented with penicillin (100 U/mL)/streptomycin (100 μg/mL), human recombinant (hr) IL-3 (30 ng/mL, only present during the first 3 weeks), hr IL-6 (50 ng/mL), and stem cell factor (SCF) (100 ng/mL). Article Title: MALAT1 promotes platelet activity and thrombus formation through PI3k/Akt/GSK-3β signalling pathway Article Snippet: Article Title: Single-cell lineage capture across genomic modalities with CellTag-multi reveals fate-specific gene regulatory changes Article Snippet: LSK cells were purified as described above, counted and 5,500 cells were added to a 96-well Article Title: NEUROBEACHIN regulates hematopoietic progenitor differentiation and survival by modulating NOTCH activity. Article Snippet: Miguel Ganuza Fernandez (Barts Centre Cancer Research UK, United Kingdom) Antonio Morales-Hernandez (University of Michigan, United States) Alanna Van Huizen (St. Jude Children's Research Hospital, United States) Ashley Chabot (St.Jude Children's Research Hospital, United States) Trent Hall (St. Jude Children's Research Hospital, ) Claire Caprio (St. Jude Children's Research Hospital, United States) David Finkelstein (SJCRH, United States) Shannon McKinney Freeman (St. Jude Children's Research Hospital, United States) Article Title: TOPO-seq reveals DNA topology-induced off-target activity by Cas9 and base editors. Article Snippet: With the increasing use of CRISPR–Cas9, detecting off-target events is essential for safety.. Current methods primarily focus on guide RNA (gRNA) sequence mismatches, often overlooking the impact of DNA topology in regulating off-target activity.. Here we present TOPO-seq, a high-throughput and sensitive method that identifies genome-wide off-target effects of Cas9 and base editors while accounting for DNA topology. Article Title: HSPA9 /mortalin inhibition disrupts erythroid maturation through a TP53 -dependent mechanism in human CD34+ hematopoietic progenitor cells Article Snippet: The cells were subsequently cultured for 5 days in Article Title: Neurobeachin regulates hematopoietic progenitor differentiation and survival by modulating Notch activity Article Snippet: HSPCs were cultured in |